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NEB助力COVID-19研究

2021年SARS-CoV-2病毒的影响依然持续，了解到您可能会对订购和接收重要研究试剂的能力感到担忧，New England Biolabs（NEB）为了支持全球客户，在努力确保我们的员工和他们的家人的安全同时，保持了我们业务的连续性。我们的生产和分销团队全线正常运转，我们与我们的供应商和分销合作伙伴密切合作，确保您能够不间断地获得我们的产品和技术支持。我们还遵循地方和国家卫生组织的指导方针，积极配合减轻COVID-19的传播。

NEB的产品仅用于研究目的。然而，我们已经在为研发更好的SARS-CoV-2病毒诊断工具和疫苗的客户提供了支持，并准备向更多的客户提供所需试剂，以验证和开发为实验室或护理点服务的诊断工具的试剂。

Select an application to learn more:

COVID_RNAExtraction_Category
COVID_VirusDetection_Category
COVID_Sequencing_Category
COVID_ViralBiology_Category
COVID_VaccineDev_Category

 

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RNA Extraction

Extraction of RNA is often the first step in viral detection assays and has become an increasingly important laboratory technique in the midst of this global pandemic. Though NEB does not offer a kit specifically designed for viral RNA extraction, both the Monarch Total RNA Miniprep Kit and the Monarch RNA Cleanup Kits have been used successfully to extract total RNA, including viral RNA, from clinically-relevant samples.

 

Monarch® Total RNA Miniprep Kit (NEB #T2010)

Developed for total RNA extraction from several sample types, this kit can also be used for viral RNA extraction.

  • Successfully used on saliva and buccal swabs as well as simulated nasopharyngeal samples
  • New streamlined protocol enables extraction from saliva in under an hour
  • Enables sensitive  downstream detection using RT-qPCR, RT-LAMP and other methods

Monarch RNA Cleanup Kits (NEB #T2040 and #T2030)

Traditionally used for cleanup applications, these kits have now been validated for extraction, expanding its utility to support viral detection.

  • Successfully used for viral RNA extraction from saliva, buccal swabs, and simulated nasopharyngeal swabs, with the addition of DNA/RNA Protection Reagent (NEB #T2011)
  • Extremely fast – extraction complete in under 10 minutes
  • Enables sensitive downstream detection using RT-qPCR, RT-LAMP and other methods

Monarch RNA purification kits can also be automated on the QIAcube® and KingFisher® Flex platforms with minor protocol modifications.

COVID_appNote_icon Purification of synthetic SARS-CoV-2 viral RNA from biological samples using the Monarch® Total RNA Miniprep Kit and the Monarch RNA Cleanup Kit
COVID_protocol_Icon RNA Extraction from Saliva, Buccal Swabs, and Nasopharyngeal Samples
COVID_protocol_Icon RNA Extraction from Saliva Using the Monarch RNA Cleanup Kits
COVID_protocol_Icon RNA Extraction from Buccal/Nasopharyngeal Swabs Using the Monarch RNA Cleanup Kits
COVID_protocol_Icon Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit
COVID_protocol_Icon Automation of Viral RNA Extraction from Saliva Using the Monarch RNA Cleanup Kit

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Virus Detection

Virus detection often utilizes nucleic acid amplification to identify the presence of specific sequences in SARS-CoV-2 viral RNA. These methods include RT-qPCR for real time quantitation and loop-mediated isothermal amplification (LAMP) for robust amplification performed at a single reaction temperature.

 

 

SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (NEB# E2019)

The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes loop-mediated isothermal amplification for use in the analysis of SARS-CoV-2, the novel coronavirus that causes COVID-19.

  • Colorimetric LAMP enables simple, visual detection (pink-to-yellow) of amplification of SARS-CoV-2 nucleic acid
  • Reduce risk of carryover contamination, with UDG and dUTP included in the master mix
  • Assay targets N and E regions of the SARS-CoV-2 genome, for optimized sensitivity and specificity
COVID_publication_Icon Many Initiatives Turning to RT-LAMP as Alternative to PCR for Rapid COVID-19 Screening Assays
COVID_publication_Icon Preprint: Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
COVID_publication_Icon Enhancing Colorimetric LAMP Amplification Speed and Sensitivity with Guanidine Chloride 
COVID_publication_Icon
 Additional citations and EUA protocols featuring colorimetric LAMP kits
SARS-CoV-2 Rapid colorimetric LAMP Assay Kit

 

WarmStart Colorimetric LAMP 2X Master Mix with UDG (NEB# M1804)

WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions.

  • Easily detect amplification with a simple pink-to-yellow color change
  • Reduce risk of carryover contamination, with UDG and dUTP included in the master mix
  • Set up reactions at room temperature, as WarmStart feature inhibits activity at 25°C
COVID_publication_Icon Many Initiatives Turning to RT-LAMP as Alternative to PCR for Rapid COVID-19 Screening Assays
COVID_publication_Icon Preprint: Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
COVID_publication_Icon Enhancing Colorimetric LAMP Amplification Speed and Sensitivity with Guanidine Chloride 
COVID_publication_Icon
 Additional citations and EUA protocols featuring Colorimetric LAMP kits
COVID_Product_WarmStartLAMP_New

 

WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800)

WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution.The mix contains a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions.

  • Easily detect amplification with a simple pink-to-yellow color change
  • Set up reactions at room temperature, as WarmStart feature inhibits activity at 25°C
  • Compatible with carryover prevention
COVID_publication_Icon Many Initiatives Turning to RT-LAMP as Alternative to PCR for Rapid COVID-19 Screening Assays
COVID_publication_Icon Preprint: Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
COVID_publication_Icon Enhancing Colorimetric LAMP Amplification Speed and Sensitivity with Guanidine Chloride 
COVID_publication_Icon
 Additional citations and EUA protocols featuring Colorimetric LAMP kits
COVID_Product_WarmStartcolorimetricLAMP

 

WarmStart LAMP Kit (DNA & RNA) (NEB# E1700)

The WarmStart LAMP Kit contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx for optimal LAMP and RT-LAMP performance. It is compatible with multiple detection methods, including real-time fluorescence detection (when used with included LAMP fluorescent dye), turbidity, and end-point visualization.

COVID_publication_Icon Many Initiatives Turning to RT-LAMP as Alternative to PCR for Rapid COVID-19 Screening Assays
COVID_publication_Icon
 Citations and protocols featuring LAMP kits
COVID_Product_WarmStartLAMPKit

 

Luna® RT-qPCR Kits

Luna Universal One-Step RT-qPCR kits offer exceptional sensitivity, reproducibility and RT-qPCR performance. Available for probe- or dye-based detection, Luna kits utilize a novel, thermostable WarmStart RT, which increases reaction specificity. Reaction mixes also contain dUTP, which supports carryover prevention. 

Relevant Luna products include:

COVID_appNote_icon Application note: Explore the latest research in direct detection of SARS-CoV-2 RNA using Luna reagents
COVID_appNote_icon
 Application note: Learn how high throughput qPCR and RT-qPCR workflows are enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB’s Luna reagents 
COVID_publication_Icon
 Additional citations and EUA protocols featuring Luna kits

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Sequencing & Epidemiology

In the COVID-19 pandemic, sequencing is enabling scientists, often in unprecedented colcovlaborations, to make rapid advances in epidemiology and surveillance, basic and clinical research, and diagnostics. A fast-growing number of methods are being developed to address these applications, including targeted SARS-CoV-2 sequencing, host genomic sequencing and metagenomic sequencing. 

 

Products for Oxford Nanopore Technologies® Sequencing

The following reagents are being recommended in a number of third party COVID-19 sequencing protocols, including “PCR tiling of COVID-19 virus” in Oxford Nanopore Technologies’ Nanopore Community, and the ARTIC protocol. 

COVID_protocol_Icon More information on these and other protocols
COVID_Product_NEBNextUltraII

 

Products for Illumina® Sequencing

A number of methods are being developed using the Illumina platform for virus, metagenomic and host sequencing, as well as diagnostics. These include ARTIC-based virus enrichment protocols and novel workflows.

  • NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770)
  • NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760)
  • NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645)
  • NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771)
  • NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB #E6111)
  • NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400)
  • LunaScript RT SuperMix Kit (NEB #E3010)
COVID_publication_Icon Additional citations featuring NEBNext products

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Viral Biology

Proteolytic cleavage between the S1 and S2 unit of the spike protein of SARS-CoV-2 is an important part of the process to allow viral entry into the host cell after binding to the surface receptor. A subsequent cleavage on the S2 unit further triggers membrane fusion. Furin is one of the key actors in a family of proteases responsible for these steps. Furin is used to study how different mutations and variations on the spike protein of SARS-CoV-2, as well as among closely related coronaviruses, respond to this proteolytic cleavage. The susceptibility of the spike protein to furin is essential to understand not only the viral biology, but also COVID-19’s transmissibility. 

The spike protein is additionally extensively glycosylated (up to 22 N-glycosylation sites). These glycans have profound implications in modulating cell entry and membrane fusion, as well as host immune response. These glycosylations can be analyzed and profiled by PNGase F, Trypsin and Endoproteinase LysC.

              

Furin (NEB # P8077)

Furin is a ubiquitous subtilisin-like proprotein convertase with a minimal cleavage site of Arg-X-X-Arg˅. The enzyme prefers the site Arg-X-Lys/Arg-Arg˅. 

  • Recombinant
  • Major processing enzyme of the secretory pathway and is localized in the trans-golgi network
  • Substrates of furin include blood clotting factors, serum proteins, growth factor receptors, such as the insulin-like growth factor receptor, and the spike protein of SARS-CoV-2
COVID_publication_Icon The sequence at Spike S1/S2 site enables cleavage by furin and phospho-regulation in SARS-CoV2, but not in SARS-CoV1 or MERS-CoV

 

Rapid PNGase F (NEB # P0710S)

Rapid PNGase F enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins. 

  • Complete deglycosylation in 10 minutes
  • Reaction components compatible with HPLC and mass spec analysis
  • Recombinant enzyme; ≥95% purity
COVID_publication_Icon Comprehensive characterization of N- and O- glycosylation of SARS-CoV-2 human receptor angiotensin converting enzyme 2
COVID_publication_Icon
 SARS-CoV-2 spike protein Interacts with multiple innate immune receptors
COVID_Product_RapidPNGaseF

Interested in using Rapid PNGase F under non-denaturing conditions? Try Rapid PNGase F (non-reducing format).

Not sure if Rapid PNGase F is the right choice? Learn more about the different PNGase F options that are available from NEB.

 

Trypsin-ultra™, Mass Spectrometry Grade (NEB # P8101S)

Trypsin-ultra, Mass Spectrometry Grade is a serine endopeptidase, which selectively cleaves peptide bonds C-terminal to lysine and arginine residues.

  • Analyze complex proteomes with minimal autolysis
  • Compatible for simultaneous co-digestion with PNGase F and endoproteinase LysC 
  • Suitable for both in-gel and solution digests

 

Endoproteinase LysC (NEB # P8109S)

Endoproteinase LysC is a serine endoproteinase, which cleaves peptide bonds at the carboxyl side of lysine. 

  • Analyze complex proteomes
  • Compatible for simultaneous co-digestion with trypsin 
  • Suitable for both in-gel and solution digests
COVID_Product_EndoproteinaseLysC

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COVID_VaccineDev_Icon

Vaccine Development

mRNA-based vaccines are an emerging alternative to conventional vaccine approaches. Unlike protein-based vaccines, mRNA vaccines involve introduction of an mRNA sequence encoding disease-specific antigens. Once delivered into the cells, they get translated by the cellular machinery, resulting in the synthesis of protein antigens. These antigens are recognized by the immune system, and immune responses are mounted. Successful mRNA vaccines for infectious diseases will prompt protective immunity from possible infection. Several mRNA vaccine candidates are in development for SARS-CoV-2. mRNA vaccines can be rapidly and scalably produced in vitro using enzymes to transcribe and modify mRNA. 

 

Reagents for mRNA synthesis

NEB’s portfolio of research-grade and GMP-grade* reagents enables bench-scale to commercial-scale mRNA manufacturing, enabling a seamless transition to large-scale therapeutic mRNA manufacturing.

COVID_info_icon Learn more about our GMP-grade in vitro transcription reagents

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Product Citation Tool

View citations that utilize NEB products using the Product Citation Tool. Refine your search by updating search field and then selecting a filter type. For your convenience, COVID-19 has been selected as a filter on this page.

 

"GMP-grade" is a branding term NEB uses to describe reagents manufactured at NEB’s Rowley facility. The Rowley facility was designed to manufacture reagents under more rigorous infrastructure and process controls to achieve more stringent product specifications and customer requirements. Reagents manufactured at NEB’s Rowley facility are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards. However, at this time, NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor does NEB manufacture its products in compliance with all of the Current Good Manufacturing Practice regulations.

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

 

 

Welcome to the COVID-19 researcher spotlight

Hear from scientists around the world as they discuss publications related to COVID-19, new protocols and techniques for detection and characterization, as well as how the scientific community is coming together to address this pandemic...all in 19 minutes or less!

In this NEB Podcast episode, Joshua Quick explains the goal of releasing the ARTIC Network’s protocol for sequencing the SARS-CoV-2 virus and how his previous experience sequencing Ebola and Zika viral outbreaks have informed these efforts.

Listen now

Past researcher spotlight topics

Catch up on previous discussions related to COVID-19 research in these podcasts and videos:

Doing COVID-19 related research?

Let us know what you are working on, so we can keep you informed on new COVID-19 related products and publications available from NEB.

Stay Informed

Interested in larger quantities?

Requests to obtain any of these products in larger quantities in connection with the COVID-19 crisis should be forwarded to bulks@neb.com.

For more general inquiries or questions, please feel free to contact us at info@neb.com.

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