| 主办单位: |
| 中国科学院遗传与发育生物学研究所 |
| 中国遗传学会 |
| 编辑单位: |
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《遗传学报》 编辑部
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| 主 编: 薛勇彪 |
| 主 任: 张颖 |
编委阵容 |
| 审稿专家 |
| 国内刊号： |
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CN 11-5450/R
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| 国际刊号： |
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ISSN 1673-8527
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| 邮发代号： 2-819
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遗传学报 2004.3 Vol 31, No.3 236-240
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| 染色体着丝粒中结构蛋白CenpB的基因 在乳腺癌组织中的活跃表达 |
| The Active Expression of CenpB,a Constitutive Protein in the Centromeres of Chromosomes,in Breast Cancer Tissues |
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| 梁前进1，鲁香凤2,程晓蕾3，罗 松1，何大澄1,①，王永潮1,① |
| 1.北京师范大学生命科学学院，北京 100875；2.北京中医药大学东直门医院，北京 100700 3.北京天安中医院，北京 100013 |
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| Key Words： 着丝粒蛋白；着丝粒蛋白B；乳腺癌 |
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Abstract
利用Northern印迹技术对31例乳腺癌组织块及每一例病人的癌旁正常（非癌性）组织块进行分析，发现多数情况下（87.1%）癌组织中着丝粒蛋白CenpB mRNA过表达；组织原位杂交分析表明，在CenpB mRNA过表达的乳腺癌组织中，呈现出比癌旁正常组织更强的杂交信号；用免疫印迹法研究同样的标本，结果与RNA水平上的研究高度一致。结果显示，CenpB基因的过表达可能与乳腺细胞恶性增殖有关。 |
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Abstract
At present,a point that cell biologists and medicine scientists focus their close attention on is the mechanisms of cell proliferation and carceration.Breast cancer,one of the frequently occurring cancers,is often been studied intensively.Centromere constitutive molecules,related to various regulatory factors,play an important role in cell proliferation check point regulation.
Cell cycle engine molecules,oncogenes,anti-oncogenes and other molecules conform a cell proliferation network.The basic courses of all tumors are associated to this network.However,there are still many problems to be resolved in the analyses of cancer related genes which cause tumors and tumor gene markers.
In the current study,using Northern blot,31 samples of breast cancer tissues and their normal (not cancerous) tissues a little far away from them in the same individuals showed that,in the majority of the tests (87.1%),the mRNA of centromere protein CenpB over expressed in breast cancer tissues,and moreover,tissue in situ hybridization also revealed that all of the CenpB-over-expressed cancer tissues,having identified with Northern blot,over expressed CenpB mRNA .Analyzing the same samples by means of Western blot,the result was highly consistent to the studies in the RNA level.A conclusion was drawn that the over expression of CenpB gene probably relates to malignant cell proliferation in breast gland.
It has been testified by researchers that a few of CenpB homogenous proteins are co-operative,the loss of their genes resulting in chromosomes' separating abnormally and cell growth's slowing down.Having transfected HeLa（Tet-Off）cells with anti-sense Cenp in a previous experiment,we ever got a result that cellular duplicating time was prolonged for another 32.8 h,and together with the inhibition of centromere assembly,the mitotic index dropped sharply.In another research,we drew a conclusion that CenpG may be related to cancer，and its differential expressing probably relates to malignant cell proliferation.Combined with these researches,the results obtained from the current study are beneficial to further recognition of the mechanism of cancer. |
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中国科学院遗传与发育生物学研究所
