In order to study the expression pattern of rice gl utelin endosperm specific promoter in Chinese cultivar Zhonghua 8 (Oryza sativ a L.subsp japonica),UidA gene was fused with rice glutelin G luA-2 gene 5′upstream sequence 2.3 kb and 750 bp upstream respe c tively and transferred into rice by Agrobacterium mediated transformation.So uthern blot indicated that UidA gene was integrated into the genome of transgenic plants as singl e copy.Northern blot demonstrated that the expression of UidA gene and endog enous GluA-2 gene reached their highest level at 13 ～15 days and 11～13 days after pollination respectively,and then declined.Histo chemical staining of immature transgenic rice seeds showed UidA gene was spe cifically expressed in endosperm and the highest level GUS expression was observ ed in aleurone layer.Quantitative analysis of GUS activity showed seeds GUS acti vity of that 2.3 kb transgenic plant was about two to three folds of those of 7 50 bp transgenic plant.Sequence analysis suggested that the G-box located in the －2 170 bp (from transcription start site) may be a quantitative cis-element.
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中国科学院遗传与发育生物学研究所
