| 主办单位: |
| 中国科学院遗传与发育生物学研究所 |
| 中国遗传学会 |
| 编辑单位: |
|
《遗传学报》 编辑部
|
| 主 编: 薛勇彪 |
| 主 任: 张颖 |
编委阵容 |
| 审稿专家 |
| 国内刊号： |
|
CN 11-5450/R
|
| 国际刊号： |
|
ISSN 1673-8527
|
| 邮发代号： 2-819
|
|
|
|
|
 |
遗传学报 2004.3 Vol 31, No.3 251-257
|
 |
| 仔猪腹泻抗性相关特异表达ESTs的筛选 |
| Screening Specifically Expressed ESTs Related to Piglet Diarrhea Resistance |
| |
| 亓小红1,张 勤1,①,刘培琼2,宋春云1,徐如海1 |
| 1.中国农业大学动物科技学院，北京 100094 2.贵州大学动物科学学院，贵阳 550001 |
| |
| Key Words： 仔猪腹泻；抗病性；DDRT-PCR；EST；信号转导蛋白；长散布元件反转录酶 |
| |
|
| |
| PDF全文 |
| |
|
| |
Abstract
以久仰香猪、剑白香猪和长白猪3个品种的脾脏为材料，以品种间同一组织不同的发病状态为对照，将DDRT-PCR技术与银染法相结合进行差异显示研究，筛选与仔猪腹泻抗性相关的特异表达ESTs。在脾脏未发病RNA池中检测到5条特异表达ESTs，其中一条与Nck转导蛋白同源，一条与长散布元件反转录酶同源，一条为相似性较高的未知功能序列，两条为新序列。 |
| |
Abstract
RNA was extracted from spleens of diarrhea and non-diarrhea piglets of Jiuyang Pig,Jianbai Pig and Landrace.RNA pools were established and DDRT-PCR using three anchored and seven arbitrary primers combined with silver staining was conducted to identify ESTs differentially expressed in diarrhea resistance to E.coli K88.Five cDNA were identified in the non-diarrhea RNA pools.Among them two are new sequences and three are highly identical to the ESTs in the GeneBank,of which two have known functions.One is the mammalian Nck adaptor protein 1 and the other is the human LINE-1 reverse transcriptase. |
| |
| |
| |
| PDF全文 |
| |
|
|
|
|
|
.gif){kind=small}
中国科学院遗传与发育生物学研究所
