| 利用重组自交系群体检测水稻条纹叶枯病 抗性基因及QTL分析 |
Abstract
Rice stripe disease transmitted by small brown plan thopper (Laodelphax striatellus Fall.) is one of the most serious viral dise ases in East Asia.The disease is severely epidemic in most rice growing areas where the main cultivars are susceptible or moderately susceptible to rice stripe virus.In this researc h,a recombinant inbred lines (RILs) population of 81 lines derived from a cross of Kinmaze (japonica)/DV85(indica) by the single seed descent method wa s used t o detect quantitative trait loci (QTL) conferring resistance to rice stripe viru s(RSV).The response of the two parents and 81 RILs to RSV were investigated by inoculating seedlings with viruliferous small brown planthopper insects,and sco red by the disease rate index.The quantitative trait loci for rice stripe disea se resistance were analyzed by QTL Cartographer software.Three QTL controlling RSV resistance were detected on chromosomes 1,7 and 11,respectively.Individual QTL accounted for 19.8%～30.9% of the phenotypic variance in the RILs popula tion.The direction of the additive gene effects at two loci qStv7 and qStv11 coincided with that predicted by phenotypes of the parents.At these two loci,the DV85 alleles increased the resistance to RSV,whil e at qStv1 ,the Kinmaze alleles increased the resista nce to RSV. |
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中国科学院遗传与发育生物学研究所
