| 鸡瘦蛋白受体（OBR）基因内含子8单核苷酸 多态性与体脂性状的相关研究 |
Abstract
Leptin receptor plays an important role in leptin f unctioning signal transduction and it may have direct effects on the deposition of adipose tissues and the body weight,the leptin receptor (OBR) gene,theref ore,can be considered as a candidate gene in the study of fat deposition of the chicken.The function of OBR gene has been intensively studied in mammals,but study of OBR gene in the chicken is still rare.In this paper,the NEAU diver gent selection broiler l ines for abdominal fat were used.Body weight and fatness traits were measured i n the sixth generation broiler population of the two lines at 7 week of age.Two pairs of primers for intron 8 of OBR gene were designed according to the da ta base of chicken genomic sequence (Accession No.AF222783).The SNP was detected by DNA sequencing,and PCR-SSCP method was then developed to screen the population .The correlation analysis between the polymorphisms of the intron 8 of OBR g ene and growth and fatness traits in the population was carried out using the ap propriate statistical model.Two SNPs were found in the population.Those were T50 0C and G659A.The least square analysis showed that BB genotype birds had sig nificant higher (P＜0.05) abdominal fat weight and percentage of abdominal fat than AA and AB genotype birds,and AA genotype birds had signific ant lower (P＜0.05) weights of livers than AB and BB genotype birds at the same time.From these results we can putatively drew the conclusion that OBR gene may be a major gene to affect the fatness traits or linked to the m ajor gene,and the two polymorphisms found in OBR gene intron 8 region could be used to select the chicken for low abdominal fat in molecu lar marker-assisted selection programs. |
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中国科学院遗传与发育生物学研究所
