| 主办单位: |
| 中国科学院遗传与发育生物学研究所 |
| 中国遗传学会 |
| 编辑单位: |
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《遗传学报》 编辑部
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| 主 编: 薛勇彪 |
| 主 任: 张颖 |
编委阵容 |
| 审稿专家 |
| 国内刊号： |
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CN 11-5450/R
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| 国际刊号： |
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ISSN 1673-8527
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| 邮发代号： 2-819
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遗传学报 2004.3 Vol 31, No.3 299-304
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| 与杨树木材密度、纤维性状相关的SSR分子标记 |
| SSR Molecular Markers Related to Wood Density and Fibr e Traits in Poplar |
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| 黄烈健，苏晓华①，张香华，黄秦军 |
| 中国林业科学研究院林业研究所，北京 100091 |
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| Key Words： 杨树；木材密度；纤维性状；SSR |
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Abstract
通过对美洲黑杨（Populus deltoides）×青杨（P.catha yana）的68个F2、双亲及其4个F1无性系田间随机区组设计，5次重复，分析了与木材 材性有关的木材密度、纤维长、纤维宽以及微纤丝角等性状。结果表明：纤维长有明显的杂 种优势，控制该性状表现的基因之间具有正效应的互作；控制木材基本密度的基因之间具有 负效应的互作。结合SSR分子标记结果，采用单因素方差分析法进行标记与性状的相关分析 ，共找到与木材密度、纤维长、纤维宽以及微纤丝角相关联的标记分别为5、7、4、2个；青 杨在这4个材性性状方面也具有对性状起贡献的标记，如与木材密度相关的PMGC2873-1（贡 献率4.88%）标记，与纤维长相关的PMGC456-3（贡献率22.96%）、PMGC2702-2（贡献率 9.17%）标记，与纤维宽相关的PMGC2408-1（贡献率7.18%）标记，与微纤丝角相关PMGC2 525-1（贡献率16.59%）标记，在育种中如果能正确加以利用，也可起到一定的材性改良 作用。 |
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Abstract
In this study,field experiment was carried out following the randomized complete block design with five replications in 1999.F2 populat ion was derived from a cross between the female ZH3 and the male ZH1 (ZH3,ZH1 we re selected from F1 individuals derived from the cross of Populus deltoides with P.cathayana,produced by HUANG Dong-Sen in 1973).Sixty-eight F2 p opulations,four F1 individuals (included ZH3,ZH1) and both parents (Populus deltoides and P.cathayana) were measured for wood densities,fibre lengths, fibre widths and fibre angles.The results indicated that the trait of fibre leng th has obviously het erosis,there may have positive effects among the genes controlling the trai t of fibre length,but may have negative effects among the genes controlling the t rait of wood density.Using the method of single factor variance,the SSR markers correlated with wood density,fibre length,fibre width and fibre angle were ident ified to be 5,7,4 and 2,respectively.There were some useful markers among the ab ove traits in P.cathayana,such as PMGC2873-1 for wood density (its contribu tion was 4.88%),PMGC456-3,PMGC2702-2 for fibre length (their contribution wer e 22.96% and 9.17%,respectively),PMGC2408-1 for fibre width (its contribution was 7.18%),and PMGC2525-1 for fibre angle (its contribution was 16.59%),thes e markers might be useful for wood property improvement if using them correctly in breeding program. |
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中国科学院遗传与发育生物学研究所
