| 多头绒泡菌PSCL32.5蛋白的性质及其含量 在细胞周期中的变化 |
Abstract
Crude fraction of Arg/Ser-rich proteins (SR protei n s) were isolated from plasmodia of Physarum polycephalum and immunoassayed b y western blot with a monoclonal antibody against SC35 protein from HeLa cell.Tw o polypeptides were detected by the antibody,suggesting that they were SCL(SC35 -like) proteins.The SCL proteins have their mass weight of 32.5 kD and 82.5 k D,respectively,and so were termed PSCL32.5 and PSCL82.5 in this paper.The PI o f PSCL32.5 was ascertained as 6.19 by IEF after a further purification of the protein with SDS-PAGE.The densitometric scanning of the western blot bands of PSCL32.5 isolated at different phases of cell cycle of P.polycephalum demon strated that the relative content of the protein varied through the cell cycle:i t appeared as the lowest at early S phase,showed increases from S phase to G2 phase,and peaked at late G2 phase. |
.gif){kind=small}
中国科学院遗传与发育生物学研究所
