| 用变性PAGE-银染法鉴定小麦抗条锈基因 Yr5的RAPD标记 |
Abstract
Abstract: RAPD analysis was performed between a near-isogeni c line (NIL) Yr5 /6×Avocet S carrying the resistance gene Yr5 against wheat stripe rust and its suscepti ble parent Avocet S,using the Yr5 gene donor parent Triticum spelta album as control.Amplified DNA fragments were separated on 4 % denaturing PAGE (polyacrylamide gel electrophoresis) and displayed by silver s taining.Fifty to 100 bands were detected,5 folds more than those revealed on aga rose g els.A total of 240 random primers were screened,and 23 reproducible polymorphic DNA fragments were found,out of which 6 polymorphic bands appeared to be linked to Yr5 gene.Genetic linkage was tested on 121 segreg ating F2 plants derived from a cross between Avocet S and Yr5 /6×Avocet S.It was showed that the polymorphic DNA fragment S1320 207 was completely linked to Yr5 gene,and S1348 363 closely linked to Yr5 gene.The results sug gested that using denaturing PAGE-silver staining could increase the level of D NA polymorphisms detected in wheat and also improve the repeatability of RAPD a nalysis. |
.gif){kind=small}
中国科学院遗传与发育生物学研究所
