In order to understand stress-inducible expression characteristics of sorbitol-6-phosphate dehydrogenase (S6PDH) promoter (S6PDHp) from apple, a series of 5' deletions of S6PDH promoter were fused with GUS reporter gene by Gateway and introduced into arabidopsis byagrobacterium-mediated transformation. The expression characteristics of S6PDHp were validated by GUS activity changed in transgenetic Arabidopsis against low temperature and exogenous ABA. Molecular detection analysis revealed that four 5' deletions of S6PDHp were fused with GUS reporter gene successfully (i.e. pGWB433-S6PDHp1, pGWB433-S6PDHp2, pGWB433-S6PDHp3 and pGWB433-S6PDHp4) and relevant transgenetic Arabidopsis were obtained. In low temperature treatment, GUS activity in pGWB433-S6PDHp3 transgenetic line increased markedly, while there were obvious change in other deletion transgenetic lines. In exogenous ABA treatment, GUS activity in others deletions transgenetic lines increased dramatically except pGWB433-S6PDHp4. These results suggest that S6PDHp can be induced by low temperature or exogenous ABA, however, there were different response in every deletions. These indicated that positive and negative cis-regulatory elements in response to abiotic stress may co-existed in the promoter region (-2 396bp to -236bp) of S6PDH.