首页 > 核心刊物 > 中国生物工程杂志 2017.6 Vol 37, No.6 > Fas凋亡抑制分子FAIM 1表达缺失诱发单纯性肥胖的初步研究

Fas凋亡抑制分子FAIM 1表达缺失诱发单纯性肥胖的初步研究

李艳伟, 马义, 韩磊, 肖兴, 党诗莹, 文涛, 王得华, 范志勇

1. 暨南大学细胞生物学系 暨南大学生物医药研究院 广东省生物工程药物重点实验室 基因工程药物国家工程研究中心 广州 510632 2. 广州中医药大学第二附属医院<广东省中医院> 广州 510405

 
Key Words:  高脂饮食; 胰岛素受体β; 临床血液; 肥胖; Fas凋亡抑制分子
 

Abstract

利用肥胖患者血液和肥胖动物模型研究Fas凋亡抑制分子FAIM 1与单纯性肥胖的关系,为揭示单纯性肥胖发生的分子机理和诊治肥胖提供新的实验基础。检测40例单纯性肥胖患者和17例正常者血液白细胞的FAIM 1蛋白的表达量,分析FAIM 1与单纯性肥胖的相关性。为进一步揭示FAIM 1与单纯性肥胖发生、发展的内在关系,利用高脂饲料建立肥胖模型,并测定肥胖组和对照组大鼠的体重和血糖水平;造模成功的肥胖组大鼠和对照组大鼠禁食12h,麻醉心脏取血,分析血脂中总胆固醇(TC)、甘油三酯 (TG)、高密度脂蛋白-胆固醇(HDL-C)和低密度脂蛋白-胆固醇(LDL-C)的变化;取肥胖组和对照组大鼠的附睾脂肪垫和肾周脂肪,分析重量变化;取肥胖组和对照组大鼠,利用Western-blot 检测FAIM 1及胰岛素受体β(IR β)的表达变化。实验结果表明:与正常者相比,单纯性肥胖患者血液白细胞的FAIM 1表达量平均减少36.4%;与对照组相比,肥胖组大鼠血清TC、TG和LDL-C分别增加37.1%、25.6%和39.1%,而HDL-C则降低33.3%;肥胖组大鼠附睾脂肪垫和肾周脂肪分别是对照组的1.85倍和2.24倍;与对照组相比,肥胖组大鼠肝脏FAIM 1、IRβ的表达量分别降低45.9%和32.6%,与临床血液样本的检测结果一致。研究表明,FAIM 1表达与单纯性肥胖发生具有显著的负相关性,FAIM 1可成为单纯性肥胖诊断和治疗的新靶点。


 

Abstract

The relationship between Fas apoptosis inhibitory molecule FAIM 1 and simple obesity was studied by using the blood of obese patients and the model of obese rats to provide the new experimental basis for revealing the molecular mechanism of obesity and the diagnosis and treatment for obesity. The correlation between FAIM 1 expression and simple obesity was determined through testing the expression of FAIM 1 protein in white blood cells for 17 cases of normal individuals and 40 cases of obese patients. To further reveal the relationship between FAIM1 and the occurrence and development of simple obesity, the model of obese rats was established by using high-fat diet, and the body weight and blood glucose levels of obese and control rats were measured. The successfully constructed obese model rats and normal rats were fasted for 12 hours. Blood taken from heart were used to measure the change of total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) after anesthetizing animals. The epididymal fat pad and perirenal fat weights of obese rats and control rats were analyzed. The expression changes of FAIM 1 and insulin receptor beta (IR β) protein were detected by Western blot. The results showed the expression of FAIM1 protein in white blood cells of patients with simple obesity was reduced by 36.4% compared with normal control. Compared with the normal control rats, the serum levels of TC, TG and LDL-C in the obese model group were respectively increased by 37.1%, 25.6% and 39.1%, while HDL-C was reduced by 33.3%. The epididymal fat pad and perirenal fat weights of the obese model rats were respectively 1.85- and 2.24-fold of that in the normal control rats. Compared with the control group, the expression of FAIM 1 and IR β in the liver of obese rats were decreased by 45.9% and 32.6%, respectively, which was consistent with the results of clinical blood samples. So it displayed that the expression of FAIM 1 is significantly nagtively correlated with simple obesity, and FAIM 1 may be a new target for diagnosis and treatment for simple obesity.

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主办单位:
中国生物工程学会(CSBT)、中国生物技术发展中心(CNCBD)、中国科学院文献情报中心。
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最新一届编辑委员会由国家生物技术研究开发计划的评审专家及有关院士组成,代表中国生物技术的最高学术水平。
编委会主任:陈志南
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国内刊号:CN11-4816/Q
国际刊号:ISSN1671-8135
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