QIAGEN News online archive:

November 2007:

e19	Interview with Eva Veronesi at the Institute of Animal Health in Pirbright
e18	First DNA forensic conviction — 20th anniversary
e17	Validation of universal conditions for duplex real-time RT-PCR assays using the QuantiTect Multiplex PCR Kit
e16	Partners for protein structural determination
e15	Characterization of protein-RNA complexes using QIAGEN Ni-NTA Magnetic Agarose Beads

October 2007:

e14	Screening the ‘epigenome’ using RNAi

September 2007:

e13	Proteins and the QIAcube
e12	Post-PCR cleanup can greatly enhance the sensitivity of STR profiling for low-copy-number casework applications
e11	Interview with Terese Bergfors, Dept. of Cell and Molecular Biology, Uppsala University, Sweden

August 2007:

e10	The world’s largest siRNA validation project
e9	High-throughput RNAi screening reveals potential cancer therapy target

July 2007:

e8	Interview with Dr. Natasha Caplen, National Cancer Institute, Maryland, USA, about the findings from her recent paper
e7	Interview with Dr. Peter M. Vallone at the National Institute of Standards and Technology
e6	Interview with Dr. Corrie Brown — avian influenza training in Afghanistan

June 2007:

e5	Let’s twist again!

May 2007:

e4	Ni-NTA — The gold standard for His-tagged protein purification
e3	The key to getting more protein — Expression screening

April 2007:

e2	DNA methylation analysis is now easier than ever!

March 2007:

--	Carrier RNA may increase extraction efficiency for certain forensic sample types

February 2007:

e1	The role of biomarkers in molecular diagnostics — current trends and future potentials

September 2006:

e14	Real-time RT-PCR for analysis of gene knockdown by RNAi - controls and calculations
e13	Interview with scientists at the San Diego Wild Animal Park
e12	Interview with Jack Ballantyne of the National Center for Forensic Science
e11	Interview with Vanessa Lynch of the Leigh Matthews Trust

August 2006:

e10	Transfection reagent toxicity in CNS primary cells minimized using Effectene Transfection Reagent

June 2006:

e7	An interview with Dr Thomas Parsons, Director of Forensic Science at The International Commission on Missing Persons
e6	An interview with Stéphane Pronost, Chef du service R&D and Dr Guillaume Fortier, Directeur of the Département Santé Animale at the Laboratoire Departmental Frank Duncombe

May 2006:

e5	The Qproteome GlycoArray Kit ・fast, comprehensive analysis of glycosylated recombinant proteins

March 2006:

e4	Automated purification of DNA from bones of a Bronze Age family using the BioRobot EZ1 workstation

January 2006:

e3	Development of a quantitative, real-time PCR assay for the detection of Epstein-Barr virus DNA in transplant patients
e2	Easy and efficient automated DNA purification from a variety of evidence samples for forensic casework
e1	Purification of parvovirus B19 DNA in performance studies of the QIAamp DSP Virus Kit for in vitro diagnostics

December 2005:

e15	QIAGEN provides essential tools for rapid and reliable detection of avian flu virus

November 2005:

e14	Highly effective siRNA design using an artificial neural network

October 2005:

e13	Routine use of the BioRobot EZ1 in detection of veterinary and zoonotic pathogens
e12	Efficient siRNA transfection and low cytotoxicity allow study of nitric oxide-mediated effects in primary endothelial cells

August 2005:

e11	siRNA successfully used as an anti-SARS therapeutic agent in Rhesus macaque

July 2005:

e10	RNAi screening of human kinases and phosphatases identifies novel targets for anticancer therapies

June 2005:

e9	Analysis of the Ras/MEK/Erk mitogenic pathway using RNAi and Redistribution assay technology
e8	A genomewide perspective of gene expression ・integration of QIAGEN RNAi technologies and Affymetrix GeneChip Arrays
e7	Reliable purification of viral RNA from all 6 HCV genotypes for in vitro diagnostics
e6	Successful automation of a high-throughput molecular biology workflow using an integrated BioRobot 8000 system
e5	Plasmid-based gene therapy for human bladder cancer
e4	The Influence of DNA Methylation on Glucose Metabolism in Cancer

May 2005:

--	High-throughput RNAi by reverse transfection with low siRNA concentrations
e3	In vivo RNAi

January - March 2005:

e2	Comparison of a range of approaches for RNAi in human cells
e1	Automated viral nucleic acid purification minireview: Efficient purification for a wide range of viruses and sample types

November 2004:

e33	QIAGEN simplifies DNA methylation research!
e32	Highly efficient gene silencing using 2-for-Silencing siRNA Duplexes targeting protein kinase genes

September 2004:

e31	LiquiChip technology facilitates activity profiling of Ser/Thr and Tyr kinases
e30	Microsatellite analysis of wild boar populations in Portugal by multiplex PCR

August 2004:

e29	Rapid Mutation Screening by Multiplex PCR and LiquiChip Suspension Array
e28	Ultra-high–throughput gene silencing for rapid, economical functional genomics studies
e27	Automated purification of recombinant proteins — high purity and yields from micro- to large-scale preparations

July 2004:

e26	Critical factors in amplification of DNA from samples that contain inhibitory substances
e25	Automated purification of mycoplasma DNA from respiratory samples
e24	Automated purification of anthrax DNA from soil for biodefense applications
e23	Easy setup of LiquiChip interaction assays using 6xHis-tagged proteins

June 2004:

e22	Getting started in RNAi research
e21	Automated purification of DNA from urine for sensitive PCR of Chlamydia trachomatis amplicons

May 2004:

e20	Alexa Fluor labeled siRNA is highly effective for monitoring transfection efficiency
e19	Easy-to-use automation for RNA purification from cells and tissues
e18	RNAi optimization and control experiments using the RNAi Human/Mouse Control Kit and QuantiTect Gene Expression Assays
e17	High PCR efficiency enables more accurate quantification in real-time PCR

April 2004:

e16	Easy and efficient automated DNA preps from reference and differentially extracted forensic samples
e15	Rapid, optimized transfection of plasmid DNA using Effectene Transfection Reagent
e14	Easy and rapid establishment of multiplex PCR assays
e13	Efficient DNA and siRNA transfection of primary cells

March 2004:

e12	High-throughput purification of DNA from plant tissues
e11	Disruption and homogenization of starting materials for purification of RNA
e10	Stable 8-year storage of DNA purified with the QIAamp DNA Blood Mini Kit
e9	6xHis tag minireview: further novel applications of QIAexpress technology
e8	General considerations for the storage of sample material prior to DNA purification

February 2004:

e7	Highly Sensitive and Specific Detection of Anaplasma phagocytophilum in Infected Cultured Cells
e6	Recombinant-protein solubility screening using the EasyXpress in vitro translation system
e5	Gene silencing of PML reduces the expression of TopBP1 after exposure of cells to ionizing radiation
e4	Efficient disruption of multiple tissue samples for RNA purification

January 2004:

e3	High-performance DNA from forensic samples using the EZ1 DNA Tissue Kit together with the EZ1 DNA Forensic Card protocols
e2	Prognostic use of circulating plasma nucleic acid concentrations in patients with acute stroke
e1	Effective gene silencing: a comparison of siRNAs designed using the QIAGEN 4-for-Silencing and Tuschl algorithms

December 2003:

e49	Analysis of protein interactions between components of the NF-κB signaling pathway using the LiquiChip system
e48	General considerations for purification of genomic DNA
e47	QIAGEN plasmid purification technologies for high-throughput transfection screening using automated and manual processing
e46	Higher sensitivity and specificity using the QIAGEN OneStep RT-PCR Kit

November 2003:

e45	Functional yields of DNA from blood with varying white cell counts using the BioRobot M48 workstation and MagAttract technology
e44	Specialized RNA purification methods for reliable downstream real-time RT-PCR
e43	Analysis of protein preparations from the BioRobot Protein LS System using the Agilent 2100 bioanalyzer
e42	Fully licensed 6xHis-tag technology for streamlined protein science procedures

October 2003:

e41	Additional protocols for specialized applications using QIAGEN products
e40	High-performance PCR template DNA from preserved blood samples using MagAttract technology and the BioRobot M48 workstation
e39	Fully automated multiplex cytokine assays
e38	Monitoring transfection efficiency and gene silencing using fluorescent labels

September 2003:

e36	Isolating RNA from long-term-stored dried blood spots
e35	Fast and easy covalent immobilization of capture biomolecules in xMAP assays
e34	Highly efficient in vitro protein expression using plasmid DNA purified with the QIAprep Miniprep System
e33	Optimizing RNAi experiments using QIAGEN siRNA and the Agilent 2100 bioanalyzer

August 2003:

e32	Automated DNA preps from soft-tissue biopsies and mouse tails using the BioRobot EZ1 system
e31	Critical factors for successful gene expression assays
e30	The serine protease Omi/HtrA2 regulates apoptosis by binding XIAP through a Reaper-like motif
e29	Reproducible high yields of high-quality DNA using MagAttract DNA Blood M48 Kits on the BioRobot M48 workstation

July 2003:

e28	Exoproteolytic affinity-tag removal using the TAGZyme System — fast and accurate analysis with the Agilent 2100 bioanalyzer
e27	Fully automated setup and measurement of xMAP sandwich ELISAs
e26	Post-transcriptional regulation of soluble guanylyl cyclase expression in rat aorta
e25	The QIAGEN Guide to Transfection, Part II: Transfection of RNA
e24	Microarray experiments using very small amounts of RNA

June 2003:

e23	Isolation of plasmid DNA from mycobacteria
e22	A comprehensive array tool for studies of cell-signaling pathways
e21	Efficient purification of high-quality DNA from blood samples with a range of white cell counts using the BioRobot EZ1 System
e20	High-throughput DNA purification from buccal swabs for use in forensic applications

May 2003:

e19	Coronavirus interview with Prof R Hilgenfeld
e18	Stabilization of RNA prior to isolation
e17	Recent developments in quantitative, real-time PCR for molecular diagnostics
e16	Fully automated isolation of genomic DNA from buccal swabs

April 2003:

e15	Highly sensitive detection of herpes simplex virus using quantitative, real-time PCR
e14	Stabilization of gene expression profiles in blood after phlebotomy
e13	Prenatal genotyping from cell-free fetal DNA in maternal plasma, using multiplex, real-time PCR
e12	Testing for trisomy 21 using quantitative real-time PCR
e11	Walkaway purification of nucleic acids is easier than ever

March 2003:

e10	Integrated, automated system for efficient gene silencing
e9	Highly efficient multiplex PCR using novel reaction chemistry
e8	Critical factors for successful cDNA labeling for microarray analysis
e7	Efficient purification of DNA from turtle shells

February 2003:

e6	Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
e5	Successful PCR amplification and subcloning of a GC-rich DNA fragment
e4	Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
e3	The QIAGEN Guide to Transfection, Part I: Introduction to transfection

January 2003:

e2	Increased sensitivity with the HiLight Array System
e1	Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent

　

QIAGEN News complete Issues:

2004		2005		2006
Issue No. 1 (pages 1-28) Issue No. 2 (pages 29-55) Issue No. 3 (pages 56-83)		Issue No. 1 (pages 1-31) Issue No. 2 (pages 32-64) Issue No. 3 (pages 65-96)		Issue No. 1 (pages 1-32) Issue No. 2 (pages 33-64) Issue No. 3 (pages 65-88) Issue No. 4 (pages 89-112)

2003		2002		2001
Issue No. 1 (pages 1-24) Issue No. 2 (pages 25-48) Issue No. 3 (pages 49-72)		Issue No. 5 Issue No. 4 Issue No. 3 Issue No. 2 Issue No. 1		Issue No. 5 Issue No. 4 Issue No. 3 Issue No. 2 Issue No. 1

　