作者简介:
Dr. Brian Eric Hall
Senior Scientist, Merck Millipore, USA
Speaker Biography:
Dr.Brian Hall is a senior scientist at Amnis Corporation and has been working on the ImageStream technology for the past 15 years. As one of the original Amnis team members, he's gained the experience of developing the hardware systems from a prototype, helped write the technical manuals, designed the training courses and conduced seminars and poster presentations the world over. He has 4 patents and over 18 publications using quantitative image Cytometry and continues to collaborate with researchers developing novel applications for the ImageStream. He is a graduate of Boston University, and spent the early part of his career doing cancer research at both the Fox Chase Cancer Research Center in Philadelphia and the Fred Hutchinson Cancer Center in Seattle. After specializing in Flow Cytometry and confocal microscopy he moved to Amnis.

内容简介:
Topic: “Protein Aggregation and Applications using the ImageStreamHigh Content Analyzer”
Seminar Abstract:
The ImageStream is a multispectral imaging flow cytometer that generates high resolution images of cells at a rate of 1000's of cells per second. This allows for the rapid acquisition of tens of thousands of images per sample. Using the IDEAS image analysis software, the system calculates features based not only on fluorescence intensity but the morphology of that fluorescence as well. With this novel approach the ImageStream is able to seamlessly combine the quantitative power of flow cytometry with the high content information associated with microscopy. The system can collect data on a wide range of applications including nuclear localization during a signal transduction cascade, measuring the apoptotic index and imaging of rare cell populations. New assay development also allows for the enumeration of protein aggregates and silicon oil droplets, a critical quality control assay for protein based pharmaceuticals and reagents.

预览幻灯片

索取Amnis成像流式细胞仪资料>>