生物通报道：《Nature》杂志下载排行榜虽然不能完整代表科学意义或者内容的引用影响，但是也许能提醒在过去的一个月里读者可能忽视的一些问题。

在上一个月的下载排名中，部分由于诺贝尔奖的原因，有关RNAi的文章占据了几项主要的新闻及报道。

No.1——Letters to Nature
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegansAndrew 
Fire et al.Nature 391, 806-811 (19 February 1998) 10.1038/35888
Abstract | Full Text | PDF

这是一篇具有划时代意义的文章，许多有关RNA干扰基础研究和技术方面的综述都会引用这篇文章，之所以重新又得到了大家的关注热点，成为下载榜单上的第一名，想来是由于2006年诺贝尔医学/生理奖引发了这一经典发现的回温。

让我们再来回顾一下这篇文章的摘要：
Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an endogenous gene. Such effects have been proposed to result from a simple antisense mechanism that depends on hybridization between the injected RNA and endogenous messenger RNA transcripts. RNA interference has been used in the nematode Caenorhabditis elegans to manipulate gene expression. Here we investigate the requirements for structure and delivery of the interfering RNA. To our surprise, we found that double-stranded RNA was substantially more effective at producing interference than was either strand individually. After injection into adult animals, purified single strands had at most a modest effect, whereas double-stranded mixtures caused potent and specific interference. The effects of this interference were evident in both the injected animals and their progeny. Only a few molecules of injected double-stranded RNA were required per affected cell, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.

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