华人学者《Nature》解析信号转导

　　

生物通报道，加利福尼亚大学旧金山分校Pao-Tien Chuang教授等人在Nature在线版上发表新见解，文章主要解析了Hedgehog信号通路的新进展，文章标题为：Fused has evolved divergent roles in vertebrate Hedgehog signalling and motile ciliogenesis。

 

Pao-Tien Chuang教授在哺乳动物发育相关信号通路研究领域建树颇丰，他的研究组致力于研究Hedgehog信号通路在早期胚胎发育和出生后在肿瘤、干细胞中的作用。

 

Hedgehog基因是一种分节极性基因，因突变的果蝇胚胎呈多毛团状，酷似受惊刺猬而得名。已知该基因编码一种高度保存的分泌型糖蛋白，对于调节果蝇胚胎发育中细胞定向分化有重要作用。

 

在这篇文章中，研究小组以Hedgehog介导的小鼠Fu（也称Stk36）突变体为研究对象，发现Fu能与Kif27相互作用，对脊椎动物的central pair apparatus的形成具有重要作用。研究小组还对斑马鱼的Fu进行研究。

 

研究结果表明，在central pair apparatus的形成过程中有一种新的信号传递路径，Hedgehog信号是调节的主要因素之一，这些成果为Hedgehog的级联反应提供了新视野。

（生物通 小茜）

 

生物通推荐原文检索：Fused has evolved divergent roles in vertebrate Hedgehog signalling and motile ciliogenesis

【Abstract】

Hedgehog (Hh) signalling is essential for several aspects of embryogenesis1, 2. In Drosophila, Hh transduction is mediated by a cytoplasmic signalling complex3, 4, 5 that includes the putative serine-threonine kinase Fused (Fu) and the kinesin Costal 2 (Cos2, also known as Cos), yet Fu does not have a conserved role in Hh signalling in mammals6, 7. Mouse Fu (also known as Stk36) mutants are viable and seem to respond normally to Hh signalling. Here we show that mouse Fu is essential for construction of the central pair apparatus of motile, 9+2 cilia and offers a new model of human primary ciliary dyskinesia. We found that mouse Fu physically interacts with Kif27, a mammalian Cos2 orthologue8, and linked Fu to known structural components of the central pair apparatus, providing evidence for the first regulatory component involved in central pair construction. We also demonstrated that zebrafish Fu is required both for Hh signalling and cilia biogenesis in Kupffer's vesicle. Mouse Fu rescued both Hh-dependent and -independent defects in zebrafish. Our results delineate a new pathway for central pair apparatus assembly, identify common regulators of Hh signalling and motile ciliogenesis, and provide insights into the evolution of the Hh cascade.