CRISPRЧȷ¼Nature vs.Scienceͷͬʱget¼

С ʱ䣺20190614 Դͨ

༭Ƽ

о˷CRISPRҪȱ㣬Ϊ򹤳̺ͻṩһǿѡ

ͨCRISPR÷ϣҲڸеѰЧӦЧʵ͡󶥼־ScienceNatureֱ𹫲Broadоŷо͸ױǴѧSam Sternbergо飨մӼݴѧУJennifer DoudnaʵתױǴѧƵоɹϸԾ򣬽DNAоȷزиDNA

о˷CRISPRҪȱ㣬Ϊ򹤳̺ͻṩһǿѡ

Ծֽתӣ޴ڣÿЯЩDNAУתøһλáһλãʵϣнӽһԾɵġ

ŷогһתøǽΪCASTCRISPRתøCRISPR-associated transposaseSternbergо¼ΪINTEGRATEInsertion of transposable elements by guide RNA-assisted targetingԾDNAвиDNA

ĿǰĹ߾ӼиDNAʵʱ༭ϸDNA޸ɵģSternbergʿ˵ ܵϸ֧䣬ġ

¼ӽǷӼ

DNAѣϸ޸ѣINTEGRATEֱڻľȷλϲûDNAУǷѧҼʮһֱѰ

еCRISPR̫

Ŀǰı༭޸ϸĻ飬ʹִ༭һ޴ĵԼ뷨

ͨоԱϣһضDNAһСĸı䣬ಿֱֲ䡣ĿǰõĹߣCRISPR-CasϵͳضиDNAӵһıӶ䡣

Щʵֻǿͷʵʵġ༭ϸDNA޸ɵģȻоԱṩDNAհס

ϸ޸екܴľԡϸ޷ȷ޸DNAѻ󣬴⣬DNAѻDNA˷ӦӰ졣

ˣʹijЩϸеĻ༭ѻ򲻿ܣоԱȫ뾫ȷŴΡ

ȡó롢óϸ

CASTϵͳ

ŷCASTϵͳCas9пøD10AżDNAתøTnpAϣȻڴ󳦸˾м⵽ֵ׸ܹٽԴDNAĶϣ˵תӿʵֻ룬DNAģƱڵͻ⡣

оԱֱ˲ͬCASTͲͬȵtracrRNACASTϵͳĻԵӰ졣4CASTԴDZģͬʱ216 bptracrRNAԲԴϣǻ֤ʵֻֻһΣӶ˶λ롣

CASTϵͳǼ򵥵޲ǴͷʼƣӴȻѰĽӶͻԭϵͳƿʵ˻ЧʵĿԽʽ

INTEGRATEϵͳ

INTEGRATEϵͳԻһ

SternbergΪҵµĻ༭ߣѰϸҵһDNA༭ϵͳһϵͳвѰԣҪϸİ

Ƿֽתϵϸеضλ㣬õøת飬ǽDNAиøøDNAλȫCRISPRϵͳơ

оԱһִһֻ༭ߣκDNAвϸκλ㡣CRISPRһøͨRNAҵʵλ㣬ȷƹDNAϵλáͨDNAЧ滻תУ10,000вϸСˣϵı༭߲ͬINTEGRATEΪֹоĵһȫɱ̵IJϵͳ

оԱڱ༭ϸв֤ʵINTEGRATEʵ˾ȷ룬ڷĿλûжĿ

Ľ༭

ܹ㷺»༭ᡣ\ƷƺϸƷ̻ƼҪȷϡ

INTEGRATEҲṩһȫµķCRISPR-Cas9ͬĿɱԺԣûDNAصĸá

ǿԶCRISPRתϵͳб̣ϵκλλ㣬֮ͨ˽乤ԭǽܹƸЧϵͳSternberg˵

һSternbergŶӽϸͣ鶯ϸSternberg˵гֵžȷDNAڲ鶯ϸ𵽺ʹ󳦸˾һãΪоյٴӦôµĴš

ͨƣ

ӭȡҵóСϸ

ԭı⣺

Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration,





˵
0      0 ˴β
¼ ע
ˢ
鿴 > >
ͨ΢Źں
΢
΢
  • Ƹְλ
  • нְλ

֪ҵƸ

ȵ

ר

ͨҳ | ն̬ | ̳ͨ | ˲г | Ŀ | ؼר |

Ȩ ͨ

Copyright© eBiotrade.com, All Rights Reserved

ϵ䣺

ICP09063491